Chlorine Identification in Rice Samples through a Method Validation Approach using Cyclic Voltammetry with Platinum Electrodes
DOI:
https://doi.org/10.22487/j24775185.2022.v11.i3.pp190-196Keywords:
Reduction reaction, hypochlorite ions, chloride ions, electrolyte, phosphate buffer solution, uncertaintyAbstract
Determination of chlorine in rice samples with a method validation approach using cyclic voltammetry based on platinum electrodes has been conducted. This study aimed to determine whether the cyclic voltammetry method with platinum electrodes is acceptable and can be recommended for routine chlorine testing in the laboratory. The basis of the analysis is the reduction reaction of hypochlorite ions to chloride ions. The initial step taken by optimizing the electrolyte with the optimal electrolyte for testing is a pH 7 phosphate buffer solution. Method validation parameters consist of determining linearity, detection limit, quantitation limit, repeatability, recovery, and measurement uncertainty. The range of standard solutions used for testing was from 10 mg/L to 150 mg/L and analyzed at a rate of 0.1 V/s. Linearity is determined based on the calculation of the coefficient of determination (R2) of 0.998, while the limits for detection and quantization are 7.49 mg/L and 24.96 mg/L, respectively. Repeatability is determined by calculating the relative standard deviation with a result of 1.77% while for recovery it is obtained at 88.19%. The result of the chlorine content test for rice samples was 0.011% with a measurement uncertainty value of 0.0011%. The major contributor to uncertainty came from the concentration of the calibration curve plot of 87.91%.
References
Astuti, R., Thessalona, I., & Setiyawan, D. T. (2020). The product quality improvement: an example from a rice milling in Indonesia. Proceeding of International Conference of Sustainability Agriculture and Biosystem (pp. 1-14). Bogor: IOP Conference Series: Earth and Environmental Science.
Bennett, K., & Zion, H. (2005). Metrology concepts: understanding test uncertainty ratio (tur). Retrieved May, 2021, from: https://www.transcat.com/media/pdf/TUR.pdf.
Bunyanis, F., Lidiawati, D., & Hawasiah. (2021). Identification of chlorine content in mantao bread sold in the city of Parepare. Journal of Global Research in Public Health, 6(1), 21-24.
Chan, C. C., Lee, Y. C., Lam, H., & Zhang, X. (2004). Analytical method validation and instrument performance verification. Hoboken: New Jersey, John Wiley & Sons.
Ellison, S. L. R., & Williams, A. (2012). Quantifying uncertainty in analytical measurement (third edition). UK: Eurachem/Citac.
Dutta, A., & Saunders, W. P. (2012). Comparative evaluation of calcium hypochlorite and sodium hypochlorite on soft-tissue dissolution. Journal of Endodontics, 38(10), 1395-1398.
Guarda, A., Aramendía, M., Andrés, I., García-Ruiz, E., Nascimento, P. C. D., & Resano, M. (2017). Determination of chlorine via the CaCl molecule by high-resolution continuum source graphite furnace molecular absorption spectrometry and direct solid sample analysis. Talanta, 162(January), 354-361.
Horwitz, W., & Albert, R. (2006). The horwitz ratio (horrat): A useful index of method performance with respect to precision. Journal of AOAC International, 89(4), 1095-1109.
Kabir, H., Ma, P. Y., Renn, N., Nicholas, N. W., & Cheng, I. F. (2019). Electrochemical determination of free chlorine on pseudo-graphite electrode. Talanta, 205(December), 1-5.
Lara-Almazán, N., Zarazúa-Ortega, G., Ávila-Pérez, P., Barrera-Díaz, C. E., & Cedillo-Cruz, A. (2021). Validation and uncertainty estimation of analytical method for quantification of phytochelatins in aquatic plants by UPLC-MS. Phytochemistry, 183(March), 1-9.
Mazurek, I., Skawińska, A., & Sajdak, M. (2021). Analysis of chlorine forms in hard coal and the impact of leaching conditions on chlorine removal. Journal of the Energy Institute, 94(February), 337-351.
Moberg, L., & Karlberg, B., (2000). An improved n,n′-diethyl-p-phenylenediamine (DPD) method for the determination of free chlorine based on multiple wavelength detection. Analytica Chimica Acta, 407(1-2), 127-133.
Mohamed, R., Zainudin, B. H., & Yaakob, A. S. (2020). Method validation and determination of heavy metals in cocoa beans and cocoa products by microwave assisted digestion technique with inductively coupled plasma mass spectrometry. Food Chemistry, 303(January), 1-6.
Palme, H., & Zipfel, J. (2022). The composition of Cl chondrites and their contents of chlorine and bromine: Results from instrumental neutron activation analysis. Meteoritics & Planetary Science, 57(2), 317-333.
Sammulia, S. F., Marliza, H., & Siahaan, A. E. (2020). Identifikasi zat klorin (Cl) dalam beras putih (oryza sativa) yang beredar di kota Batam. Jurnal Sains dan Teknologi Pangan, 5(3), 2878-2885.
Sekimoto, S., & Ebihara, M. (2017). Accurate determination of chlorine, bromine and iodine in US geological survey geochemical reference materials by radiochemical neutron activation analysis. Geostandards and Geoanalytical Research, 41(2), 213-219.
Slaughter, R. J., Watts, M., Vale, J. A., Grieve, J. R., & Schep, L. J. (2019). The clinical toxicology of sodium hypochlorite. Clinical Toxicology, 57(5), 303-311.
Taverniers, I., Loose, M. D., & Bockstaele, E. V. (2004). Trends in quality in the analytical laboratory II analytical method validation and quality assurance. Trends in Analytical Chemistry, 23(8), 535-552.
Thompson, M., Ellison, S. L., & Wood, R. (2002). Harmonized guidelines for single-laboratory validation of methods of analysis. IUPAC Pure and Applied Chemistry, 74(5), 835-855.
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