Development of Katuk Leaf Dietary Supplement for Stunting Prevention Using an In Silico Method
DOI:
https://doi.org/10.22487/j24775185.2026.v15.i1.pp32-38Keywords:
Docking stunting, sauropus androgynous, tissue pharmacologyAbstract
Stunting is a chronic nutritional problem caused by long-term malnutrition, resulting in growth disorders in children. This study aims to predict the mechanism of action of secondary metabolites of katuk leaves as an alternative treatment for growth disorders in children (stunting). This research used network pharmacology to predict compounds from katuk leaves (Sauropus androgynus) based on literature studies. Network pharmacology analysis of katuk leaf (Sauropus androgynus) compounds identified 19 proteins, with HSP90AA1 having the highest degree, 14. This study uses molecular docking to predict interactions between active compounds, such as butyrolactone, phenol, and 2,3,4-trimethylpyrrole, and NAD-dependent protein deacetylase sirtuin-1, Aldo-keto reductase family 1 member C3, Carbonic anhydrase 5A, mitochondrial, Cyclin-dependent-like kinase 5, and Glucocorticoid receptor. The molecular docking results show that the butyrolactone compound has the highest binding affinity for 3 proteins, including NAD-dependent protein deacetylase sirtuin-1, with a binding affinity of -4.7 kcal/mol. The phenol compound has 3 proteins with the highest binding affinity, including Aldo-keto reductase family 1 member C3 at -5.5 kcal/mol. And the compound 2,3,4-trimethylpyrrole has 3 proteins with the highest binding affinity values, including the Glucocorticoid receptor, with a value of -5.8 kJ/mol.
References
Afifah, F. (2024). Metabolite profiling of kenikir leaf extract (cosmos caudatus) using UPLCMS/MS and network pharmacology methods to determine molecular mechanisms in the treatment of colon cancer. Unpublished undergraduate’s thesis. Malang: Universitas Islam Negeri Maulana Malik Ibrahim.
Anjani, N. L. A. K., & Laksmiani, N. P. L. (2022). Potential of isoquercitrin as an antihyperpigmentation agent in silico using molecular docking method. Proceedings of the National Pharmacy Workshop and Seminar (pp. 171–181). Bali: Universitas Udayana. https://doi.org/10.24843/WSNF.2022.v01.i01.p14
Annisa, A. B. S., Sukmawaty, E., & Masriany, M. (2024). Prediksi komputasi potensi bioaktivitas sauropus androgynus dengan platform Way2drug. Filogeni: Jurnal Mahasiswa Biologi, 4(1), 64–71. https://doi.org/10.24252/filogeni.v4i1.45385
Arif, S., Isdijoso, W., Fatah, A. R., & Tamyis, A. R. (2020). Strategic review of food and nutrition security in Indonesia. Jakarta: SMERU Research Institute.
Artika, R. J. A. (2024). The effect of radio frequency current and antibiotics on the growth of escherichia coli bacteria and wound treatment in mice (mus mucullus) with diabetes mellitus. Unpublished undergraduate’s thesis. Malang: Universitas Islam Negeri Maulana Malik Ibrahim.
Arumsari, W., Supriyati, D., & Sima, P. (2022). Evaluation of the implementation of the stunting prevention program in the covid-19 pandemic era. Indonesian Journal of Health Management, 10(2), 82–94. https://doi.org/10.14710/jmki.10.2.2022.82-94
Arwansyah, A., Lewa, A. F., Muliani, M., Warnasih, S., Mustopa, A. Z., & Arif, A. R. (2023). Molecular recognition of moringa oleifera active compounds for stunted growth prevention using network pharmacology and molecular modeling approach. ACS omega, 8(46), 44121–44138. https://doi.org/10.1021/acsomega.3c06379
Aulia, K. P., Hidayat, N. S., Ahmad, R. K. M., Heryadi, D., & Sari, D. S. (2025). Kerja sama Indonesia dan UNICEF dalam rangka menuju Indonesia bebas stunting tahun 2021-2025. Padjadjaran Journal of International Relations, 7(2), 197–209. https://doi.org/10.24198/padjirv7i2.60585
Chen, D., Oezguen, N., Urvil, P., Ferguson, C., Dann, S. M., & Savidge, T. C. (2016). Regulation of protein-ligand binding affinity by hydrogen bond pairing. Science advances, 2(3), 1-16. https://doi.org/10.1126/sciadv.1501240
Hamdy, M. K., Rustandi, H., Suhartini, V., Koto, R. F., Agustin, S. S., Syifa, C. A., Arhabi, A., Baskara, V. A., Refiandinova, F., & Syauqy, A. (2023). The Role of posyandu cadres in reducing stunting rates. Jurnal Ilmu Sosial Indonesia, 4(2), 87–96. https://doi.org/10.15408/jisi.v4i2.37128
Hizriyani, R. (2021). Exclusive breastfeeding as a prevention of stunting. Bunda Window Journal, PG-PAUD Study Program, Muhammadiyah University of Cirebon, 8(2), 55–62. https://doi.org/10.32534/jjb.v8i2.1722
Ibrahim, I. A., Alam, S., Adha, A. S., Jayadi, Y. I., & Fadlan, M. (2021). Socio-cultural relationship with stunting incidence in toddlers aged 24-59 months in Bone-Bone Village, Baraka District, Enrekang Regency in 2020. AL GIZZAI: Public Health Nutrition Journal, 1(1), 16–26. https://doi.org/10.24252/algizzai.v1i1.19079
Jadhav, P. A., Thomas, A. B., Pathan, M. K., Chaudhari, S. Y., Wavhale, R. D., & Chitlange, S. S. (2025). Unlocking the therapeutic potential of unexplored phytocompounds as hepatoprotective agents through integration of network pharmacology and in-silico analysis. Scientific Reports, 15(1), 1–29. https://doi.org/10.1038/s41598-025-92868-y
Jaitak, A., Kumari, K., Kounder, S., & Monga, V. (2024). Carbonic anhydrases: Moiety appended derivatives, medicinal and pharmacological implications. Bioorganic & Medicinal Chemistry, 114(November), 1–36. https://doi.org/10.1016/j.bmc.2024.117933
Khaerunnisa, S., Suhartati., & Awaluddin, R. (2020). In silico research for beginners. Surabaya: Airlangga University Press.
Kiwandono, A. A., Rinawati, R., Suharso, S., Sembiring, Z., Nurhasanah, N., Anisa, D. N., Afriyani, H., & Aini, F. N. (2023). Mentoring and socialization of the use of family medicinal plants (toga) in Rejomulyo village. TABIKPUN: Jurnal Pengabdian Kepada Masyarakat, 4(1), 47–54. https://doi.org/10.23960/jpkmt.v4i1.112
Kurczab, R., Kucwaj-Brysz, K., & Śliwa, P. (2019). The significance of halogen bonding in ligand–receptor interactions: The lesson learned from molecular dynamic simulations of the D4 receptor. Molecules, 25(1), 1–14. https://doi.org/10.3390/molecules25010091
Mukminin, A. U. (2024). Molecular docking study of bitter melon fruit compounds (momordica charantia l.) of the flavonoid group as antidiabetic at the ppar receptor. Unpublished undergraduate’s thesis. Malang: Universitas Islam Negeri Maulana Malik Ibrahim.
Mulyani, A. T., Khairinisa, M. A., Khatib, A., & Chaerunisaa, A. Y. (2025). Understanding stunting: impact, causes, and strategy to accelerate stunting reduction—a narrative review. Nutrients, 17(9), 1–29. https://doi.org/10.3390/nu17091493
Murti., Hidayah, N., Marini, E., & Handayani, L. (2024). Permen seksi ratu ceting (persiapan menyusui asi eksklusif dengan sayur katuk cegah stunting pada ibu hamil). Faedah: Jurnal Hasil Kegiatan Pengabdian Masyarakat Indonesia, 2(4), 104–110. https://doi.org/10.59024/faedah.v2i4.1100
Nasir, B., Serdiati, N., Tangkesalu, D., Lasmini, S. A., Taiyeb, A., & Yudana, K. (2024). Training on making and applying biorational pesticides for environmentally friendly pest control: supporting green economy in Sigi Regency, Central Sulawesi. Proceedings of the National Seminar on Community Service, Ma Chung University, 5 (pp. 37–47). Jawa Timur: Universitas Ma Chung.
Penning, T. M., Wangtrakuldee, P., & Auchus, R. J. (2019). Structural and functional biology of aldo-keto reductase steroid-transforming enzymes. Endocrine reviews, 40(2), 447–475. http://dx.doi.org/10.1210/er.2018-00089
Pinard, M. A., Mahon, B., & McKenna, R. (2015). Probing the surface of human carbonic anhydrase for clues towards the design of isoform specific inhibitors. BioMed Research International, 2015(1), 1–15. http://dx.doi.org/10.1155/2015/453543
Prakosa, G. G. (2023). Forest mapping and basic geographic information systems. Malang: UMM Press.
Pujiastuti, N., Antarsih, N. R., Apriningsih., Suriani, H., & Faridha, N. (2023). Budidaya tanaman sauropus androgynus (daun katuk) untuk bahan olahan pangan ibu menyusui. Masyarakat Berdaya dan Inovasi, 4(1), 10–15. https://doi.org/10.33292/mayadani.v4i1.109
Sapriana., Nurhardianti., & Akbar. (2025). Evaluasi program penanganan stunting di Desa Kessing Kecamatan Donri-Donri Kabupaten Soppeng. Jurnal Ners, 10(1), 1398–1405. https://doi.org/10.31004/jn.v10i1.53083
Sari, I. W., Junaidin., & Pratiwi, D. (2020). Molecular docking study of flavonoid compounds of cat's whiskers herb (orthosiphon stamineus b.) on α-glucosidase receptors as type 2 antidiabetics. Farmagazine Journal, 7(2), 54–60. http://dx.doi.org/10.47653/farm.v7i2.194
Sari, N. P., Nurrachmi, I., Jamilah, L., Nurliawati, N., Zaeni, M. R., & Nasawi, A. A. (2025). Pertukaran informasi terhadap penanganan stunting di Indonesia dan Korea Selatan. Jurnal Pengabdian Masyarakat: Pemberdayaan, Inovasi, dan Perubahan, 5(5), 178–184. https://doi.org/10.59818/jpm.v5i5.1816
Schiedel, M., Marek, M., Lancelot, J., Karaman, B., Almlöf, I., Schultz, J., Sippl, W., Pierce, R. J., Romier, C., & Jung, M. (2015). Fluorescence-based screening assays for the NAD+-dependent histone deacetylase smSirt2 from Schistosoma mansoni. Journal of Biomolecular Screening, 20(1), 112–121. https://doi.org/10.1177/1087057114555307
Suherman, M., Prasetiawati, R., & Fauzi, I. (2022). Uji in silico senyawa aktif daun katuk (sauropus androgynus l. merr.) sebagai antiobesitas. Garut: Fakultas MIPA UNIGA
Sulistyani, S., Annasiyah, S., Khasanah, N., Setyowati, R., Zusnita, I., Lisjarwati., & Sofiyanti, I. (2024). Literatur review: Faktor-faktor yang memengaruhi terjadinya stunting pada anak. Prosiding Seminar Nasional dan Call for Paper Kebidanan (pp. 462–468). Jawa Tengah: Universitas Ngudi Waluyo
Widyastuti, R., Tari, A. I. N., & Asmoro, N. W. (2020). Aktivitas antioksidan teh daun ketapang (terminalia catappa). Jurnal Ilmu Pangan dan Hasil Pertanian, 4(2), 220–227. https://doi.org/10.26877/jiphp.v4i2.7468
Wössner, N., Alhalabi, Z., González, J., Swyter, S., Gan, J., Schmidtkunz, K., Zhang, L., Vaquero, A., Ovaa, H., Einsle, O., Sippl, W., & Jung, M. (2020). Sirtuin 1 inhibiting thiocyanates (S1th)—A new class of isotype selective inhibitors of NAD+ dependent lysine deacetylases. Frontiers in Oncology, 10(April), 1–15. https://doi.org/10.3389/fonc.2020.00657
Yang, X., Yang, H., Shen, D., Zhang, Q., & Zhu, C. (2025). Bioinformatics identification of key genes and therapeutic targets for exercise intervention in polycystic ovary syndrome. Frontiers in Genetics, 16(November), 01–12. https://doi.org/10.3389/fgene.2025.1634588
Yusuf, M. N. S., Tandiabang, P. A., & Rasdianah, N. (2023). Edukasi tepat sasaran sebagai upaya percepatan penurunan insidensi stunting di Desa Meranti, Bone Bolango. Jurnal Pengabdian Masyarakat Farmasi: Pharmacare Society, 2(1), 22–28. https://doi.org/10.37905/phar.soc.v2i1.18580
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