Profile Secondary Metabolite Compounds and Antioxidant Activities of Stem Bark Jambu Mete (Anacardium occidantale L.) Extract
DOI:
https://doi.org/10.22487/j24775185.2021.v10.i3.pp133-138Keywords:
Antioxidant, extraction, flavonoids, free radicals, stress oxidativeAbstract
Oxidative stress is a condition in which the amount of free radicals and antioxidants in the body is not balanced, causing various diseases. One of them is a degenerative disease. Oxidative stress can be reduced by antioxidant compounds. Anacardium occidentale L. is a type of plant from the Anacardiaceae family. This plant is found in many trophic countries included Indonesia, and is believed to ward off degenerative diseases caused by oxidative stress. The purpose of this study was to analyze the activity of A. occidentale stem bark extract against DPPH radical inhibition (1,1-Diphenyl-2-Picrilhidrazil) and the profiling of secondary metabolites in their active fraction. The bark of A. occidentale was extracted by a gradient maceration method. The solvents used were n-hexane (nonpolar), ethyl acetate (semi-polar), and methanol (polar). The macerate was concentrated using a rotary vacuum evaporator, followed by DPPH radical inhibition analysis using a spectrophotometer. The most active extract was analyzed for chemical content by LC-MS/MS. The results showed that the extract of A. occidentale stem bark had antioxidant activity. Methanol extract obtained very strong and best antioxidant activity with an IC50 value of 47.13 ppm compared to ethyl acetate extract and n-hexane extract, which had IC50 values of 163.92 ppm and 501.72 ppm, respectively. Secondary metabolites that are thought to have antioxidant activity in the methanol extract of A. occidentale stem bark were epigallocatechin (EC) and epigallocatechin gallate (EGCG) compounds.
References
Arief, H., & Widodo, M. A. (2016). Peranan stres oksidatif pada proses penyembuhan luka. Jurnal Ilmiah Kedokteran Wijaja Kusuma, 5(2), 22-29.
Anwar, Y. A. S. (2017). Antioxidant activity of cashew apple dreg extract and their effect in traditional processing of coconut oil. ALCHEMY Jurnal Penelitian Kimia, 13(1), 17-28.
Arifin, B., & Ibrahim, S. (2018). Struktur, bioaktivitas dan antioksidan flavonoid. Jurnal Zarah, 6(1), 21-29.
Arnanda, Q. P., & Nuwarda, R. F. (2019). Review Artikel: Penggunaan radiofarmaka teknesium-99m dari senyawa glutation dan senyawa flavonoid sebagai deteksi dini radikal bebas pemicu kanker. Farmaka, 17(2), 236-243.
Carolus, F. P., Fatimawali., & Wewengkang, D. S. (2014). Uji efektifitas ekstrak kulit batang jambu mete (Anacardium occidentale L.) terhadap penurunan kadar glukosa darah pada tikus putih jantan galur wistar (Rattus norvegicus) yang diinduksi aloksan. Pharmacon: Jurnal Ilmiah Farmasi, 3(3), 204-210.
Dahlia, A. A., & Hasnawati. (2014). Isolasi dan identifikasi golongan kimia aktif antioksidan ekstrak etanol daun jambu mete (Anacardium occidentale L.). Jurnal Fitofarmaka Indonesia, 1(1), 24-30.
Damayanti, A., & Fitriana, E. A. (2012). Pemungutan minyak atrsiri mawar (Rose Oil) dengan metode maserasi. Jurnal Bahan Alam Terbarukan, 1(2), 1-8.
Handayani, V., Ahmad, A. R., & Sudir, M. (2014). Uji aktivitas antioksidan ekstrak metanol bunga dan daun patikala (Etlingera elatior (Jack) R. M. Sm) menggunakan metode DPPH. Pharmaceutical Science and Research (PSR), 1(2), 86-93.
Hasnaeni., Usman, S., & Wisdawati. (2019). Pengaruh metode ekstraksi terhadap rendemen dan kadar fenolik ekstrak tanaman kayu beta-beta (Lunasia amara Blanco). Jurnal Farmasi Galenika (Gelenika Journal of Pharmacy), 5(2), 175-182.
Khaira, K. (2010). Menangkal radikal bebas dengan antioksidan. Jurnal Saintek, 2(2), 183-187.
Kusumowati, I. T. D., Melannisa, R., & Ratri, K. (2011). Korelasi kandungan fenolik dan aktivitas antioksidan daun jambu mete. Biomedika, 3(2), 25-30.
Leslie, P. J., & Gunawan, S. (2019). Uji fitokimia an perbandingan efek antioksidan pada daun teh hijau, teh hitam, dan teh putih (Camellia sinensis) dengan metode DPPH (2,2-difenil-1-pikrilhidrazil). Tarumanagara Medical Journal, 1(2), 383-388.
Maesaroh, K., Kurnia, D., & Anshori, J. A. (2018). Perbandingan metode uji aktivitas antioksidan DPPH, FRAP dan FIC terhadap asam askorbat, asam galat dan kuersetin. Chimica et Natura Acta, 6(2), 93-100.
Malangngi, L. P., Sangi, M. S., & Paendong, J. J. E. (2012). Penentuan kandungan tanin dan uji aktivitas antioksidan ekstrak biji buah alpukat (Persea americana Mill.). Jurnal MIPA UNSRAT, 1(1), 5-10.
Molyneux, P. (2004). The use of the stable free radical diphenylpicrylhydrazil (DPPH) for estimating antioxidant activity. Songklanakarin Journal of Science and Technology, 26(2), 212-219.
Nailufa, Y., & Najih, Y. A. (2020). Formulasi krim epigallocatechin sebagai anti-aging. Journal of Pharmacy and Science, 5(2), 81-85.
Narmada, I. B., Sarasati, A., Wicaksono, S., Rezkita, F., Wibawa, K. G. P., Hayaza, S., & Nugraha, A. P. (2020). Phytochemical screening, antioxidant activity, fuctional groups and chemical element characterization anaysis of (-)-epigallocatechin-3- gallate (EGCG) in east javanese green tea methanolic extract: An experimental in vitro study. Systemic Reviews in Pharmacy, 11(5), 511-519.
Notariza, K. R., & Krisnamurti, D. G. B. (2019). Perbandingan aktivitas antioksidan campuran ekstrak etanol A. indica dan C. asiatica terhadap ekstrak etanol A. indica. e-Journal Kedokteran Indonesia, 5(2), 98-104.
Nurfadillah., Chadijah, S., & Rustiah, W. (2016). Analisis antioksidan ekstrak etil asetat dari kulit buah rambutan (Nephelium lappaceum) dengan menggunakan metode DPPH (1,1 difenil-2-pikrilhidrazil). Al-Kimia, 4(1), 78-86.
Pratiwi, L., Fudholi, A., Martien, R., & Pramono, S. (2016). Ekstrak etanol, ekstrak etil asetat, fraksi etil asetat dan fraksi n-heksan kulit manggis (Garcinia mangostana L.) sebagai sumber zat bioaktif penangkal radikal bebas. Journal of Pharmaceutical Science and Clinical Research, 1(1), 71-82.
Priandi, F., Yusro, F., Diba, F., Mariani, Y., & Nurhaida. (2019). Uji efektifitas antibakteri ekstrak kulit batang jambu monyet (Bellucia pentamera Naudin) terhadap pertumbuhan bakteri Escherichia coli dan Salmonella typhi. Jurnal Tengkawang, 9(1), 27-37.
Romadanu., Rachmawati, S. H., & Lestari, S. D. (2014). Pengujian aktivitas antioksidan ekstrak bunga lotus (Nelumbo nucifera). Jurnal Fishtech, 3(1), 1-7.
Rudiana, T., Suryani, N., Indriatmoko, D. D., Yusransyah, Amelia, A., Noviany, & Hadi, S. (2019). Characterization of oxidative fraction of plant stem Bouea macrophylla Griff. Journal of Physics: Conference Series, 1314, 1-8.
Sang, S., Cheng, X., Stark, R. E., Rosen, R. T., Yang, C. S., & Ho, C. T., (2002). Chemical studies on antioxidant mecanism of tea catechin: Analysis of radical reaction product of catechin and epicatechin with 2,2-diphenyl-1-picrylhydrazil. Bioorganic and Medicinal Chemistry, 10(7), 2233-2237.
Sinaga, F. A. (2016). Stress oksidatif dan status antioksidan pada aktivitas fisik maksimal. Jurnal Generasi Kampus, 9(2), 176-189.
Sogandi, G., Darma, W. S. T., & Jannah, R. (2019). Potensi senyawa antibakteri dari ekstrak akar manis (Glycyrrhiza glabra L.) terhadap Bacillus cereus. Jurnal Kimia Sains dan Aplikasi, 22(4), 105-111.
Soleha, F. (2018). Pengaruh metode ekstraksi maserasi terhadap aktivitas antibakteri daun sirih merah (Piper crocatum Ruiz & Pav) pada bakteri Staphylococcus aureus menggunakan metode sumur difusi. Jurnal Analis Farmasi, 3(1), 62-70.
Tangkuman, A. R. I., Fatimawali., & Citraningtyas, G. (2017). Uji aktivitas antibakteri ekstrak etanol kulit batang jambu mete (Anacardium occidentale L.) dalam menghambat pertumbuhan bakteri dari air liur penderita sariawan. Pharmacon: Jurnal Ilmiah Farmasi, 6(2), 7-13.
World Healt Organization (WHO). (2019). Monitoring healt for the sustainable development goals. Retrieved November 15, 2010, from Available:https://apps.who.int/iris/bitstream/handle/10665/311696/WHO-DAD-2019.1-eng.pdf.
Yati, S. J., Sumpono., & Candra, I. N. (2018). Potensi aktivitas antioksidan metabolit sekunder dari bakteri endofit pada daun Mongifera oleifera L. ALOTROP, Jurnal Pendidikan dan Ilmu Kimia, 2(1), 82-87.
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