Ethanol Extract Antioxidant Activity Test of Tembelekan (Lantana camara L.) using 1,1-diphenyl-2-picrylhydrazyl (DPPH) Reagent
DOI:
https://doi.org/10.22487/j24775185.2022.v11.i3.pp153-158Keywords:
Prickly lantana, antioxidant, DPPH, maceration, IC50Abstract
The plant prickly lantana (Lantana Camara L.) contains alkaloids, flavonoids, saponins, tannins, and triterpenoids and has the potential to be used as a natural antioxidant source. The research objective is to determine the IC50 value for the antioxidant activity of prickly lantana leaf extract. Prickly lantana leaves were macerated in a 96% ethanol solvent for 3x24 hours. Phytochemical analysis of prickly lantana leaves revealed that the leaves contained alkaloids, flavonoids, saponins, and tannins. This antioxidant activity test was performed using the reagent 1,1-diphenyl-2-picrylhydrazyl (DPPH), whose absorption at 517 nm was measured using a UV-Vis spectrophotometer, and by varying the sample concentration after the addition of prickly lantana leaf extract. The concentrations of prickly lantana leaf extract used varied from 20 ppm to 40 ppm, 60 ppm, and 80 ppm. The positive control was vitamin C at the same concentration variation, and the negative control was DPPH solution dissolved in 96% ethanol. The results showed that the IC50 of prickly lantana leaf extract was 164.639 ppm, while vitamin C had an IC50 of 18.754 ppm.
References
Amin, A,. Wunas, J., & Anin, Y. M. (2014). Uji aktivitas antioksidan ekstrak etanol klika faloak (Sterculia quadrifida R.Br) dengan metode DPPH (2,2-diphenyl-1-picrylhydrazyl). Jurnal Fitofarmaka Indonesia, 2(2), 111-114.
Dris, R., & Jain, S. M. (2004). production practices and quality assessment of food crops: quality handling and evaluation. New York: Springer Dordrecht.
Fajriah, S., & Megawati, M. (2015). Penapisan fitokimia dan uji toksisitas dari daun myristica fatua houtt. Chimica at Natura Acta, 3(3), 116-119.
Fauziah, A., Sudirga, S. K., & Parwanayoni, N. M. S (2021). Antioxidant test leunca plant leaf extract (solanum nigrum l.). Journal of Biological Sciences, 8(1), 28-34.
Fitriani, N., Herman., & Rijai, L. (2019). Antioksidan ekstrak daun sumpit (brucea javanica (l). merr) dengan metode DPPH. Jurnal Sains dan Kesehatan, 2(1), 57-62.
Lestari, I. P., Mappiratu, M., Ruslan, R., & Satrimafitrah, P. (2018). Uji aktivitas antibakteri daun tanaman tembelekan (lantana camara linn) dari beberapa tingkat kepolaran pelarut. KOVALEN Jurnal Riset Kimia, 4(3), 244-253.
Lung, J. S. K., & Destiani, D. P. (2017). Uji aktivitas antioksidan vitamin A, C, E dengan metode DPPH. Farmaka, 15(1), 53-62.
Mangela, O., Ridhay, A., & Musafira. (2016). Kajian aktivitas antioksidan ekstrak daun tembelekan (lantana camara l.) berdasarkan tingkat kepolaran pelarut. KOVALEN Jurnal Riset Kimia, 2(3), 16-23.
Minarno, E. B. (2015). Skrining fitokimia dan kandungan total flavonoid pada buah carica pubescens lenne & k. koch di kawasan Bromo, Cangar, dan dataran tinggi Dieng. El-Hayah Jurnal Biologi, 5(2), 73-82.
Molyneux, P. (2004). The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin Journal of Science and Technology (SJST), 26(2), 211-219.
Pakaya, W., Ischak, N. I., & Tangio, J. S. (2015). Analisis kadar flavonoid dari ekstrak metanol daun dan bunga tembelekan. Unpublished master's thesis. Gorontalo: Universitas Negeri Gorontalo.
Prakash, A. (2001). Antioxidant activity. medallion laboratories. Journal Analithycal Progress, 19(2), 1-6.
Rachmani, E. P. N., Pramono, S., & Nugroho, A. E. (2018). Aktivitas antioksidan fraksi flavonoid bebas andrografolid dari herba sambiloto (andrographis paniculata). Pharmacy Medical Journal (PMJ), 1(2), 42-49.
Rahmi, H. (2017). Review: aktivitas antioksidan dari berbagai sumber buah-buahan di Indonesia. Jurnal Argotek Indonesia, 2(1), 34-38.
Ridho, E. A., Sari, R., & Wahdaningsih, S. (2014). Uji aktivitas antioksidan ekstrak metanol buah lakum (cayratia trifolia) dengan metode DPPH (2,2-difenil-1-pikrilhidrazil). Jurnal Mahasiswa Farmasi Fakultas Kedokteran Universitas Tanjungpura, 1(1), 1-12.
Rizkayanti., Diah, A. W. M., & Jura, M. R. (2017). Uji aktivitas antioksidan ekstrak air dan ekstrak etanol daun kelor (moringa oleifera LAM). Jurnal Akademika Kimia, 6(2), 125-131.
Salamah, N., & Widyasari, E. (2015). Aktivitas antioksidan ekstrak metanol daun kelengkeng (euphoria lohan (l) steud) dengan metode penangkapan radikal 2,2’-difenil-1-pikrilhidrazil. Pharmaciana, 5(1), 25-34.
Sami, F. J., & Rahim, S. (2015). Uji aktivitas antioksidan ekstrak metanol bunga brokoli (brassica oleracea l. var. Italica) dengan metode DPPH (2,2 diphenyl-1-picrylhydrazyl) dan metode ABTS (2,2 azinobis (3-etilbenzotiazolin)-6-asam sulfanoat). Jurnal Fitofarmaka Indonesia (JFFI), 2(2) 107-110.
Tanzaq, T. T., Agustina, R. D., Setiawati, K. E., & Cahyani, I. M. (2013). Uji aktivitas penangkapan radikal DPPH (1,1-diphenyl-2-picrylhdrazyl) ekstrak etanol kayu sacang (caesalpinia sappan l.). Media Farmasi Iindonesia, 14(1), 1461-1465.
Talapessy, S., Suryanto, E., & Yudistira, A. (2013). Uji aktivitas antioksidan dari ampas hasil pengolahan sagu (metroxylan sagu rottb). Jurnal Ilmiah Farmasi, 2(3), 40-44.
Venkatachalam T., Kumar, V. K., Selvi, P. K., Maske, A. O., & Kumar, N. S. (2011). Physicochemical and preliminary phytochemical studies on the Lantana camara (l.) fruits. International Jurnal of Pharmacy and Pharmaceutical Science, 3(1), 52-54.
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