Analysis of the Levels of Ethanol Extract Flavonoid Compounds in Cocor Bebek (Kalanchoe Pinnata) Leaves using UV - Vis Spectrophotometer
DOI:
https://doi.org/10.22487/j24775185.2023.v12.i1.pp65-70Keywords:
Flavonoids, cocor bebek leaves (kalanchoe pinnata), UV-Vis spectrophotometerAbstract
Cocor bebek is a plant that is easy to cultivate in Indonesia. This cocor bebek contains chemical compounds such as steroids, alkaloids, glycosides, tannins, and flavonoids which have antioxidant properties. Research on the analysis of flavonoid levels in the leaves of cocor bebek (kalanchoe pinnata) has been carried out using the UV-Vis spectrophotometric method. This research aims to determine the levels of flavonoids in the ethanol extract of cocor bebek leaves. The extract of chemical content in cocor bebek leaves was carried out by maceration method using 70 % ethanol. The flavonoid content of the sample extract was measured by measuring the absorption at wavelengths of 510 nm and 700 nm. The results obtained in the qualitative test showed that the cocor bebek leaf extract was positive for flavonoids which were characterized by a change in color from brownish red to green. The results of the quantitative test showed that the flavonoid content in the cocor bebek leaf extract was 0.321 mg/100 g.
References
Amina., Tomayahu, N., & Abidin, Z. (2016). Penetapan kadar flavonoid total ekstrak etanol kulit buah alpukat (persea americana mill.) Dengan metode spektrofotometri UV-VIS. Jurnal Fitofarmaka Indonesia, 4(2), 226-230.
Balakrishna, T., Vidyadhara, S., Sasidhar, R. L. C., Ruchitha, B., Prathyusha, E. V. (2016). A review on extraction techniques. Indo American Journal of Pharmaceutical Sciences (IAJPS), 3(8), 880-891.
Chaves, J. O., de Souza, M. C., da Silva, L. C., Lachos-Perez, D., Torres-Mayanga, P. C., Machado, A. P. D. F., Forster-Carneiro, T., Vázquez-Espinosa, M., González-de-Peredo, A. V., Barbero, G. F., Rostagno, M. A. (2020). Extraction of flavonoids from natural sources using modern techniques. Frontiers of Chemistry, 8(September), 1-25.
De Luna, R. S. L., Ramírez-Garza, R. E., Saldívar, S. O. S. (2020). Environmentally friendly methods for flavonoid extraction from plant material: Impact of their operating conditions on yield and antioxidant properties. The Scientific World Journal, 2020(Agustus), 1-38.
Deveoglu, R., & Karadag, R. (2019). A review on the flavonoids – a dye source. International Journal of Advances in Engineering and Pure Sciences, 31(3), 188-200.
Dias, M. C., Pinto, D. C. G. A., & Silva, A. M. S. (2021). Plant flavonoids: Chemical characteristics and biological activity. Molecules, 26(17), 1-16.
Guandjar, I. G., & Rohman, A. (2012). Drug analysis by spectrophotometry and chromatography. Yogyakarta: Student Libraries.
Karak, P. (2019). Biological activities of flavonoids: An overview. International Journal of Pharmaceutical Sciences and Research, 10(4), 1567-1574.
Khan, S., Newport, D., Le Calvé, S. (2019). Gas detection using portable deep-uv absorption spectrophotometry: A review. Sensors, 19(23), 1-31.
Khopkar, S. M. (2010). Basic concepts of analytical chemistry. Jakarta: UI Press.
Kolodziejczyk-Czepas, J., & Stochmal, A. (2017). Bufadienolides of kalanchoe species: An overview of chemical structure, biological activity and prospects for pharmacological use. Phytochemistry Review, 16(6), 1155-1171.
Kumar, S., & Pandey, A. K. (2013). Chemistry and biological activities of flavonoids: An overview. The Scientific World Journal, 2013(December), 1-16.
Mahasuari, N. P. S., Paramita, N. L. P. V., & Putra, A. A. G. R. Y. (2020). Effect of methanol concentration as a solvent on total phenolic and flavonoid content of beluntas leaf extract (pulchea indica l.). Journal of Pharmaceutical Science and Application, 2(2), 77-84.
Malik, A., Edward, F., & Waris, R. (2014). Skrining fitokimia dan penetapan kandungan flavonoid total ekstrak metanolik herba boroco (celosia argentea l.). Jurnal Fitofarmaka Indonesia, 1(1), 1-5.
Matthew, S., Jain, A. K., James, M., Matthew, C. & Bhowmik, D. (2013). Analgesic and anti-inflammatory activity of kalanchoe pinnata (lam.) pers. Journal of Medicinal Plants Studies, 1(2), 24-28.
Mattioli, R., Francioso, A., Mosca, L., & Silva, P. Anthocyanins: A comprehensive review of their chemical properties and health effects on cardiovascular and neurodegenerative diseases. Molecules, 25(17), 1-42.
Masturi., Alighiri, D., Edie, S. S., Drastisianti, A., Khasanah, U., Tanti, K. A., Susilawati., Maghfiroh, R. Z., Kirana, K. G. C., & Choirunnisa, F. (2020). Identification of flavonoid compounds and total flavonoid content from biowaste of local durian shell (Durio zibethinus). Journal of Physics: Conference Series (pp 1-11).
United Kingdom: IOP Publishing.
Minarno, E. B. (2015). Skrining fitokimia dan kandungan total flavanoid pada buah carica pubescens lenne & k. Koch di kawasan Bromo, Cangar, dan dataran tinggi Dieng. El-Hayah, 5(2), 73-82.
Neldawati., Ratnawulan., & Gusnedi. (2013). Analisis nilai absorbansi dalam penentuan kadar flavonoid untuk berbagai jenis daun tanaman obat. Pillar of Physics: Jurnal Berkala Ilmiah Fisika, 2(Oktober), 76-83.
Ngibad, K. (2019). Phytochemical screening of sunflower leaf (helianthus annuus) and anting-anting (acalypha indica linn) plant ethanol extract. Borneo Journal of Pharmacy, 2(1), 24-30.
Nugrahawati, Y. A. (2010). The effect of various temperature variations and packaging colors on anthocyanin stability. Unpublished undergraduate thesis. Surakarta: Universitas Sebelas Maret.
Panche, A. N., Diwan, A. D., & Chandra, S. R. (2016). Flavonoids: An overview. Journal of Nutritional Science, 5(e47), 1-15.
Pratiwi, R. A., & Nandiyanto, A. B. D. (2022). How to read and interpret UV-VIS spectrophotometric results in determining the structure of chemical compounds. Indonesian Journal of Educational Research and Technology, 2(1), 1-20.
Putri, A. H., Putriyana, R. S., & Silviani, N. (2019). Isolasi dan ekstraksi kelompok senyawa flavonoid dari ekstrak daun cocor bebek (kalanchoe pinnata). Fullerene Journal of Chemistry, 2(2), 28-33.
Rahmasita., Nuryanti, S., & Supriadi. (2021). Analysis of Flavonoid Levels in Tin (Ficus carica Linn) Fruit. Jurnal Akademika Kimia, 32-35.
Veitch, N. C., & Grayer, R. J. (2011). Flavonoids and their glycosides, including anthocyanins. Natural Product Reports, 28(10), 1626–1695.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2024 Ulfana Tisman, Supriadi Supriadi
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
