ANTIOXIDANT ACTIVITY TEST OF LIDAH MERTUA (SANSEVIERIA TRIFASCIATA P.) LEAVES EXTRACT USING 1,1-DIPHENIL-2-PIKRILHIDRAZIL

Rihanah Rihanah; Minarni Rama Jura

doi:10.22487/me.v16i1.735

Authors

Rihanah Rihanah Universitas Tadulako
Minarni Rama Jura Universitas Tadulako

DOI:

https://doi.org/10.22487/me.v16i1.735

Keywords:

Lidah mertua, antioxidant, DPPH, UV-Vis spectrophotometer

Abstract

Antioxidant activity test of lidah mertua (Sansevieria trifasciata P.) leaves extract using 1,1-diphenil-2-picrylhydrazyl has been carried out. The purpose of this study was to determine the antioxidant activity of ethanol extracts of old and young leaves of lidah mertua (Sansevieria trifasciata P.). The radical concentration of 1,1-diphenil-2-picrylhydrazyl (DPPH) after the addition of ethanol extracts of old and young leaves of lidah mertua was determined using a UV-Vis spectrophotometer. The concentrations used in the ethanol extracts of old and young leaves of lidah mertua were 20 ppm, 40 ppm, 60 ppm, 80 ppm and 100 ppm. The IC₅₀ values obtained of ethanol extracts of old and young leaves of lidah mertua were 131.336 ppm and 144.568 ppm respectively, while IC₅₀ value of vitamin C as a comparison was 65.433 ppm. It can be seen that the antioxidant ethanol extracts of old and young leaves of lidah mertua are moderate compared to vitamin C.

References

Achmad, S.A. (1986). Buku materi pokok kimia organik bahan alam. Jakarta: Karunika Jakarta Universitas Terbuka.

Arifin, B. & Ibrahim, S. (2018). Struktur, bioaktivitas dan aktivitas antioksidan flavonoid. Jurnal Zarah, 6(1): 21-29.

Artanti, A. N. & Lisnasari, R. (2018). Uji aktivitas antioksidan ekstrak ethanol daun family solanum menggunakan metode reduksi radikal bebas DPPH. Journal of Pharmaceutical Science and Clinical Research, 02: 62-69.

Chezem, W. R & Clay N. K. (2016). Regulation of plant secondary metabolism and associated specialized cell development by MYBs and bHLHs. Phytochemistry, 131(1): 26-43.

Estikawati, I & Lindawati, N. Y. (2019). Penetapan kadar flavonoid buah oyong (Luffa acutangula (L.) Roxb) dengan metode spektrofotometri Uv-Vis. Jurnal Farmasi Sains dan Praktis, 5(2): 96-105.

Hasnaeni & Aminah. (2019). Uji aktivitas antioksidan dan profil fitokimia ekstrak kayu beta-beta (Lunasia amara Blanco). Jurnal Farmasi Galenika, 5(1): 101-107.

Hestiningtyas, B., Siallagan, J., & Holle, E. (2019). Uji aktivitas ekstrak daun gatal (Laportea decumanum (Roxb.) Kuntze) sebagai antioksidan. AVOGADRO, 3(1): 1-5.

Ifada, R., Hermayanti, D. & Nurhasan, Y. T. (2016). Pengaruh ekstrak daun lidah mertua (Sansevieria b Trifasciata Laurentii). Jurnal FK UNISMUH Malang, 12(2): 66-74.

Kosasih, E. & Setiabudi, T. (2004). Peran antioksidan pada lanjut usia. Pusat Kajian Nasional Masalah Lanjut Usia.

Lombogia, B., Budiarso, F. & Bodhi, W. (2016). Uji daya hambat ekstrak daun lidah mertua (Sansevierae Trifasciata Folium) terhadap pertumbuhan Bakteri Esherichia coli dan Streptococcus sp. Jurnal e-Biomedik, 4(1):

Mariani, S., Rahman, N. & Supriadi. (2018). Uji aktivitas antioksidan ekstrak buah semangka (Citrullus lanatus). Jurnal Akademika Kimia, 7(2): 96-101.

Marliani, L., dkk. (2017). The effect of extraction condition on the polyphenol content and antioxidant activity of Curcuma zedoaria (Christm.) Roscoe Rhizome. IJPST, 4(2).

Masrifah, Rahman, N., & Abram, P. H. (2017). Uji aktivitas antioksidan ekstrak daun dan kulit labu air (Lagenaria siceraria (M.) Standl.). Jurnal Akademika Kimia, 6(2): 98-106.

Molyneux, P. (2004). The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. J. Sci. Technol. 26(2): 211-219.

Nasution, H. & Rahma, M. (2014). Pengujian antiradikal bebas difenilpikril hidrazil DPPH ekstrak etil asetat daun nangka (Artocapus heterophyllus Lam.). Jurnal Sains Dasar, 3(2): 137-141.

Nurhaeni, Gladys, & Hardi, J. (2019). Uji aktivitas antioksidan ekstrak lumut hati (Marchantia polymorpha). Kovalen, 5(3): 315-321.

Prakash, A., Rigelhof, F., & Miller, E., (2001). Antioxidant activity. Medallion Laboratories: Analithycal Progres, 19(4): 1-4.

Robinson, T. (1995). Kandungan organik tumbuhan tinggi. Bandung: ITB press.

Sagita, D., Aliyah, S. H., & Safitri, M. (2018). Potensi lidah mertua (Sansevieria trifasciata P.) dalam menghambat pertumbuhan bakteri Salmonella Spp dan Staphylococcus Aureus. Riset Informasi kesehatan, 7(2): 129-133.

Sari, A. K. & Ayati, R. (2018). Penentuan aktivitas antioksidan ekstrak etanol daun jeruk purut (Citrus hystrix DC) dengan metode DPPH (1,1-diphenyl-2-picrylhydrazyl). Journal Current Pharmateutical Science (JCPS), 1(2): 69-74.

Sastrohamidjojo, H. (2016). Kimia dasar. Yogyakarta: Gadjah Mada University press.

Shanmugam, S., Usha, R. V., & Pradeep, B. V. (2018). Antioxidant activity of rhizome extract of Coleus forskohlii Briq. Asian journal of pharmaceutical and clinical research,11(11): 275-279.

Sunilson et al. (2009). Analgesic and antipyretic effects of sansevieria trifasciata leaves. J. Traditional, Complementary and Alternative Medicines, 6(4): 529-533.

Susana, I., Ridhay, A. & Bahri, S. (2018). Kajian aktivitas antioksidan ekstrak batang kecombrang (Etlingera elatior) berdasarkan tingkat kepolaran pelarut. Kovalen, 4(1):16-23.

Wandita, G. A. & Musfiroh, I. (2018). Tanaman suku Zingiberaceae yang memiliki aktivitas sebagai antioksidan. Farmaka, 16(2): 564-571.

Widianingsih, M. (2016). Aktivitas antioksidan ekstrak metanol buah naga merah (Hylocereus polyrhizus (F.A.C Weber) britton & rose) hasil maserasi dan dipekatkan dengan kering angin. Jurnal Wiyata, 3(2): 146-150.

Widiastuti. (2016). Aktivitas antioksidan dan tabir surya ekstrak etanol daun stroberi (Fragaria X ananassa A.N. Duchesne). Jurnal Sains Farmasi & Klinis, 3(1), 19-24.